Commentary for Makkot 27:5

ורבנן נמי תיפוק ליה מהא אין הכי נמי ואלא כרת דאחותו למה לי לחייבו על אחותו ועל אחות אביו ועל אחות אמו

And let the Rabbis also derive it from here. Yes this is so[, everyone actually does derive the principle of separation from here]. So rather then why do the Rabbis need the verse of kares b'achoso for [if they aren't learning the principle of separation]? To make one liable [to three korban chatoses if he inadvertently cohabited in the same of lapse of unawareness] with his sister, his father's sister, and his mother's sister.

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