Commentary for Megillah 5:2

אין מהוה הוו ולא הוו ידעי הי באמצע תיבה והי בסוף תיבה ואתו צופים ותקינו פתוחין באמצע תיבה וסתומין בסוף תיבה

No, all the letters were already known, just it was not known which are the final letters and which come in the beginning or middle of words. The prophets came and established the open letters in the middle of words, and the closed at the ends of words.

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