Reference for Avodah Zarah 66:9

כסי רב אסי אסר ורב אשי שרי אי שתי בהו עובד כוכבים פעם ראשון כ"ע לא פליגי דאסור כי פליגי בפעם שני

Cups [used with wine by idolater]: R. Assi forbids, but R. Ashi permits. If an idolater drank from it the first time it was used, no one disputes that it is forbidden, they argue only if it was the second time.

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