Reference for Chullin 111:14

אינקיב עילאה אע"ג דלא אינקב תתאה ניחוש דלמא עילאה אינקיב תתאה לא אינקיב

that unless the lower membrane of the brain has also been pierced [it would not be trefah]; but according to him who says that [it is trefah] even if only the upper membrane and not the lower had been pierced, we ought to be apprehensive of this test for it might well be that the upper membrane has been pierced and the lower has not.<span class="x" onmousemove="('comment',' So that even though nothing of the brain substance exudes it might nevertheless be trefah because of the perforation of the upper membrane. Accordingly the test stated is not reliable.');">⁹</span>

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