Related for Pesachim 229:4
ואפילו למ"ד מצות אין מבטלות זו את זו ה"מ דאורייתא בדאורייתא או דרבנן בדרבנן אבל דאורייתא ודרבנן אתי דרבנן ומבטיל ליה לדאוריית'
A man must not make a sandwich of mazzah and bitter herbs together and eat them, because we hold that mazzah nowadays<span class="x" onmousemove="('comment',' I.e., after the destruction of the Temple and the cessation of sacrifices.');"><sup>4</sup></span> is a Biblical obligation, whereas bitter herbs are a Rabbinical requirement and thus the bitter herbs, which are Rabbinical, will come and nullify the mazzah, which is Biblical.
Tosefta Pesachim
The [bitter] lettuce and the matzah and the Passover offering are obligated on the first night of the [Passover] Festival, and on the remaining nights [partaking of them] is optional. Rabbi Shimon says, it is an obligation for men but optional for women. Hillel the Elder would wrap all three of them together and eat them. From what time are they eaten? They are eaten once it gets dark. [If] he did not eat them once it got dark, he may eat them [at any time during] the whole night. [If] he did not eat them [at any time during] the whole night, he cannot eat them from then on. The [bitter] lettuce and the matzah and the Passover offering -- one does not prevent fulfillment [of the obligation] of the other (e.g., if one lacks the bitter herb, he can still fulfill the mitzvot of matzah and the Passover offering).
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