Midrash for Sanhedrin 54:8
מני אילימא רבי יוסי והאיכא עדות בדיני נפשות שאין האשה כשרה לה והן כשרין לה אלא לאו רבי מאיר היא
AND ALL WHO WERERELATED AT THAT MOMENT.<span class="x" onmousemove="('comment',' When the incident which they wished to attest occurred, though they are no longer so at the time they wish to testify in court. ');"><sup>21</sup></span>
Sifrei Devarim
From (Devarim 24:16) "Fathers shall not be put to death by cause of sons, and sons shall not be put to death by cause of fathers," we learn that they (i.e., kin) do not testify against each other. We hereby infer (that to secure testimony against an inciter to idolatry) witnesses are sequestered against him.
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